Western blot analysis of SFTPD in extracts of A-549 cells. Samples were incubated with SFTPD Polyclonal antibody (Product # PA5-110362) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.